Select Publications By Category

Antibody Engineering and the MHC Class I-Related Receptor, FcRn

  • Swiercz, R., Chiguru, S., Tahmasbi, A., Ramezani, S. M., Hao, G., Challa, D. K., Lewis, M. A., Kulkarni, P. V., Sun, X., Ober, R. J., Mason, R. P., and Ward, E. S.(2014) Use of Fc-engineered antibodies as clearing agents to increase contrast during PET. J. Nucl. Med., 55, 1204—1207.
  • Kang, J. C., Poovassery, J. S., Bansal, P., You, S., Manjarres, I. M., Ober, R. J., and Ward, E. S. (2013) Engineering multivalent antibodies to target heregulin-induced HER3 signaling in breast cancer cells. mAbs, 6, 340—353.
  • Devanaboyina, S., Lynch, S. M., Ober, R. J., Ram, S., Kim, D., Puig-Canto, A., Breen, S., Kasturirangan, S., Fowler, S., Peng, L., Zhong, H., Jermutus, L., Wu, H., Webster, C., Ward, E. S., and Gao, C. (2013) The effect of pH dependence of antibody-antigen interactions on subcellular trafficking dynamics. mAbs, 5, 849—857.
  • Gan, Z., Ram, S., Ober, R. J., and Ward, E. S. (2013) Using multifocal plane microscopy to reveal novel trafficking processes on the recycling pathway. J. Cell Sci., 126, 1176—1188.
  • Patel, D. A., Puig-Canto, A., Challa, D. K., Montoyo H. P., Ober, R. J., and Ward, E. S. (2011) Neonatal Fc receptor blockade by Fc engineering ameliorates arthritis in a murine model, J. Immunol., 187, 1015—1022.
  • Montoyo, HP., Vaccaro, C., Hafner, M., Ober, R. J., Mueller, W., and Ward, E. S.(2009) Conditional deletion of the MHC class I-related receptor FcRn reveals the sites of IgG homeostasis in mice, Proc. Natl. Acad. Sci. USA, 106, 2788—2793.
  • Gan, Z., Ram, S., Vaccaro, C., Ober, R. J., and Ward, E. S. (2009) Analyses of the recycling receptor, FcRn, in live cells reveal novel pathways for lysosomal delivery, Traffic, 10, 600—614.
  • Mi, W., Wanjie, S., Lo, S. T., Gan, Z., Pickl-Herk, B., Ober, R. J., and Ward, E. S.(2008) Targeting the neonatal Fc receptor for antigen delivery using engineered Fc fragments, J. Immunol., 181, 7550—7561.
  • Prabhat, P., Gan, Z., Chao, J., Ram, S., Vaccaro, C., Gibbons, S., Ober, R. J. and Ward, E. S. (2007) Elucidation of intracellular recycling pathways leading to exocytosis of the Fc receptor, FcRn, by using multifocal plane microscopy,Proc. Natl. Acad. Sci. USA, 104, 5889—5894.
  • Vaccaro, C., Bawdon, R., Wanjie, S., Ober, R. J. and Ward, E. S. (2006)Divergent activities of an engineered antibody in murine and human systems have implications for therapeutic antibodies, Proc. Natl. Acad. Sci. USA, 103, 18709—18714.
  • Vaccaro, C., Zhou, J., Ober, R. J. and Ward, E. S. (2005) Engineering the Fc region of immunoglobulin G to modulate in vivo antibody levels, Nature Biotech., 23, 1283—1288.
  • Zhou, J., Mateos, F., Ober, R. J. and Ward, E. S. (2005) Conferring the binding properties of the mouse MHC Class I-related receptor, FcRn, onto the human ortholog by sequential rounds of site-directed mutagenesis, J. Mol. Biol., 345, 1071—1081.
  • Ober, R. J., Martinez, C., Lai, X., Zhou, J. and Ward, E. S. (2004) Exocytosis of IgG as mediated by the receptor, FcRn: an analysis at the single-molecule level, Proc. Natl. Acad. Sci. USA, 101, 11076—11081.
  • Ober, R. J., Martinez, C., Vaccaro, C., Zhou, J., and Ward, E. S. (2004) Visualizing the site and dynamics of IgG salvage by the MHC Class I-related receptor, FcRn, J. Immunol., 172, 2021—2029.
  • Zhuo, J., Johnson, J., Ghetie, V., Ober, R. J. and Ward, E. S. (2003) Generation of mutated variants of the human form of the MHC class I-related receptor, FcRn, with increased affinity for mouse immunoglobulin G, J. Mol. Biol., 332, 901—913.
  • Kim, J-K., Firan, M., Radu, C., Kim, C-H., Ghetie, V. and Ward, E. S. (1999)Mapping of the site on human IgG1 for binding of the MHC class I related receptor, FcRn, Eur. J. Immunol., 29, 2819—2825.
  • Ghetie, V., Popov, S., Borvak, J., Radu, C., Matesoi, D., Medesan, C., Ober, R. J. and Ward, E. S. (1997) Increasing the serum persistence of an IgG fragment by random mutagenesis, Nature Biotech., 15, 637—640.
  • Medesan, C., Matesoi, D., Radu, C., Ghetie, V. and Ward, E. S. (1997)Delineation of the amino acid residues involved in transcytosis and catabolism of mouse IgG, J. Immunol., 158, 2211—2217.
  • Ghetie, V., Hubbard, J. G., Kim, J-K., Tsen, M-F., Lee, Y. and Ward, E. S. (1996)Abnormally short serum half lives of IgGs in beta2-microglobulin deficient mice, Eur. J. Immunol., 26, 690—696.
  • Ward, E.S., Güssow, D., Griffiths, A., Jones, P.T. and Winter, G. (1989) Binding activities of a repertoire of single immunoglobulin variable domains secreted from Escherichia coli, Nature, 341, 544—546.

Autoimmune Disease and T Cell Biology

  • Challa, D. K., Bussmeyer, U., Khan, T., Montoyo, H. P., Bansal, P., Ober, R. J., and Ward, E. S. (2013) Autoantibody depletion ameliorates disease in murine experimental autoimmune encephalomyelitis. mAbs, 5, 655—659.
  • Bansal, P., Khan, T., Bussmeyer, U., Challa, D. K., Swiercz, R., Velmurugan, R., Ober, R. J., and Ward, E. S. (2013) The encephalitogenic, human myelin oligodendrocyte glycoprotein-induced antibody repertoire is directed toward multiple epitopes in C57BL/6 mice. J. Immunol., 191, 1091—1101.
  • Maverakis, E., Menezes, J. S., Ametani, A., Han, M., Stevens, D. B., He, Y., Wang, Y., Ono, Y., Miyamura, Y., Lam, K. S., Ward, E. S., and Sercarz, E. E.,(2010) Molecular mimics can induce a nonautoaggressive repertoire that preempts induction of autoimmunity, Proc. Natl. Acad. Sci. USA, 107, 2550—2555.
  • Pastor, S., Minguela, A., Mi, W., and Ward, E. S. (2009) Autoantigen immunization at different sites reveals a role for anti-inflammatory effects of IFN-gamma in regulating susceptibility to experimental autoimmune encephalomyelitis, J. Immunol., 182, 5268—5275.
  • Minguela, A., Pastor, S., Mi, W., Richardson, J. A., and Ward, E. S. (2007)Feedback regulation of murine autoimmunity via dominant anti-inflammatory effects of interferon gamma, J. Immunol., 178, 134—144.
  • Huang, J. C., Ober, R. J. and Ward, E. S. (2005) The central residues of a T cell receptor sequence motif are key determinants of autoantigen recognition in murine experimental autoimmune encephalomyelitis, Eur. J. Immunol., 35, 299—304.
  • Huang, J. C., Han, M., Minguela, A., Pastor, S., Qadri, A. and Ward, E.S. (2003) T cell recognition of distinct peptide: I-Au conformers in murine experimental autoimmune encephalomyelitis, J. Immunol., 171, 2467—2477.
  • Machius, M., Cianga, P., Deisenhofer, J., and Ward, E. S. (2001) Crystal structure of a T cell receptor Va11 (AV11S5) domain: new canonical forms for the first and second complementarity determining regions, J. Mol. Biol., 310, 689—698.
  • Garcia, K. C., Radu, C. G., Ho, J., Ober, R. J., and Ward, E. S. (2001) Kinetics and thermodynamics of T cell receptor-autoantigen interactions in murine experimental autoimmune encephalomyelitis, Proc. Natl. Acad. Sci. USA, 98, 6818—6823.
  • Qadri, A., Radu, C. G., Thatte, J., Cianga, P., Ober, B. T., Ober, R. J., and Ward, E. S. (2000) A role for the region encompassing the c” strand of a T cell receptor Va domain in T cell activation events, J. Immunol., 165, 820—829.
  • Thatte, J., Qadri, A., Radu, C., and Ward, E. S. (1999) Molecular requirements for T cell recognition by an MHC Class II restricted T cell receptor: the involvement of the fourth hypervariable loop of the Va domain, J. Exp. Med.,189, 509—519.
  • Fields, B. A., Ober, B., Malchiodi, E. L., Lebedeva, M. I., Braden, B., Ysern, X., Kim, J-K., Shao, X., Ward, E. S., and Mariuzza, R. A. (1995) Crystal structure of the Va domain of a T cell antigen receptor, Science, 270, 1821—1824.

Fluorescence Microscopy

  • Tahmasbi, A., Ram, S., Chao, J., Abraham, A. V., Tang, F. W., Ward, E. S., and Ober, R. J. (2014) Designing the focal plane spacing for multifocal plane microscopy. Opt. Express, 22, 16706—16721.
  • Chao, J., Ram, S., Ward, E. S., and Ober, R. J. (2013) Ultrahigh accuracy imaging modality for super-localization microscopy. Nat. Methods, 10, 335—338.
  • Gan, Z., Ram, S., Ober, R. J., and Ward, E. S. (2013) Using multifocal plane microscopy to reveal novel trafficking processes on the recycling pathway. J. Cell Sci., 126, 1176—1188.
  • Ram, S., Kim, D., Ward, E. S., and Ober, R. J. (2012) 3D single molecule tracking with multifocal plane microscopy reveals rapid intercellular transferrin transport at epithelial cell barriers. Biophys. J., 103(7), 1594-1603.
  • Ram, S., Prabhat, P., Chao, J., Ward, E. S., and Ober, R. J. (2010) Three-dimensional Single molecule tracking of quantum-dot labeled antibody molecules, Proc. SPIE., 7575, 75750J.
  • Abraham, A. V., Ram, S., Chao, J., Ward, E. S., and Ober, R. J. (2009)Quantitative study of single molecule location estimation techniques, Opt. Express, 17, 23352—23373.
  • Ram, S., Prabhat, P., Ward, E. S., and Ober, R. J. (2009) Improved single particle localization accuracy with dual objective multifocal plane microscopy,Opt. Express, 17, 6881—6898.
  • Chao, J., Ram, S., Abraham, A. V., Ward, E. S., and Ober, R. J. (2009) A resolution measure for three-dimensional microscopy, Optics Com., 282, 1751—1761.
  • Ram, S., Prabhat, P., Chao, J., Ward, E. S., and Ober, R. J. (2008) High accuracy 3D quantum dot tracking with multifocal plane microscopy for the study of fast intracellular dynamics in live cells, Biophys. J., 95, 6025—6043.
  • Prabhat, P., Gan, Z., Chao, J., Ram, S., Vaccaro, C., Gibbons, S., Ober, R. J., and Ward, E. S. (2007) Elucidation of intracellular recycling pathways leading to exocytosis of the Fc receptor, FcRn, by using multifocal plane microscopy,,Proc. Natl. Acad. Sci. USA, 104, 5889—5894.
  • Prabhat, P., Ram, S., Ward, E. S., and Ober, R. J. (2006) Simultaneous imaging of several focal planes in fluorescence microscopy for the study of cellular dynamics in 3D, Proc. SPIE., 6090, 115—121.
  • Ram, S., Ward, E. S., and Ober, R. J. (2006) Beyond Rayleigh’s criterion: A resolution measure with application to single-molecule microscopy, Proc. Natl. Acad. Sci. USA, 103, 4457—4462.
  • Lai, X., Lin Z., Ward, E. S., and Ober R. J. (2005) Noise suppression of point spread functions and its influence on deconvolution of three dimensional fluorescence microscopy image sets, J. Microscopy, 217, 93—108.
  • Ober, R. J., Martinez, C., Lai, X., Zhou, J., and Ward, E. S. (2004) Exocytosis of IgG as mediated by the receptor, FcRn: An analysis at the single-molecule level, Proc. Natl. Acad. Sci. USA, 101, 11076—11081.
  • Prabhat, P., Ram, S., Ward, E. S., and Ober, R. J. (2004) Simultaneous imaging of different focal planes in fluorescence microscopy for the study of cellular dynamics in three dimensions., IEEE Trans. Nanobio., 3, 237—242.
  • Ober, R. J., Ram, S., and Ward, E. S. (2004) Localization accuracy in single-molecule microscopy, Biophys. J., 86, 1185—1200. Please see MIATool for more information.

Signal Processing

  • Cohen, E. A. K. and Ober, R. J. (2013) Analysis of point based image registration errors with applications in single molecule microscopy, IEEE Trans. Signal Process., 61, 6291—6306.
  • Ram, S., Ward, E. S., and Ober, R. J. (2013) A stochastic analysis of distance estimation approaches in single molecule microscopy – quantifying the resolution limits of photon-limited imaging systems, Multidim. Sys. Sig. Proc.,24, 503—542.
  • Chao, J., Ward, E. S., and Ober, R. J. (2012) Fisher information matrix for branching processes with application to electron-multiplying charge-coupled devices, Multidim. Sys. Sig. Proc., 23, 349—379.
  • Wong, Y., Lin, Z., and Ober, R. J. (2011) Limit of the accuracy of parameter estimation for moving single molecules imaged by fluorescence microscopy,IEEE Trans. Sig. Proc., 59, 895—911.
  • Chao, J., Ward, E. S., and Ober, R. J. (2010) A software framework for the analysis of complex microscopy image data, IEEE Trans. Info. Tech. Biomed.,14, 1075—1087.
  • Abraham, A. V., Ram, S., Chao, J., Ward, E. S., and Ober, R. J. (2010)Comparison of estimation algorithms in single-molecule localization, Proc. SPIE., 7570, 757004.
  • Zou, Q., Lin, Z., and Ober, R. J. (2006) The Cramer Rao lower bound for bilinear systems, IEEE Trans. Sig. Proc., 54, 1666—1680.
  • Ram, S., Ward, E. S., and Ober, R. J. (2006) A stochastic analysis of performance limits for optical microscopes, Multidim. Sys. Sig. Proc., 15, 27—57.
  • Lin, Z., Zou, Q., Ward, E. S. and Ober, R. J. (2005) The Cramer Rao lower bound for parameter estimation in nonlinear systems, IEEE Sig. Proc. Letts.,12, 855—858.
  • Ober, R. J., Lai, X., Lin, Z. and Ward, E. S. (2005) State space realization of a three-dimensional image set with application to noise reduction in fluorescent microscopy images of cells, Multidim. Sys. Sig. Proc., 16, 7—48.
  • Hunt, L. R., Ward, E. S., and Ober, R. J. (2004) Approximation of trajectories of nonlinear systems by iterates of systems with linear state dynamics, Sys. Cont. Letts., 51, 377—381.
  • Ober, R. J., Lin, Z., and Zou, Q. (2003) Calculations of the Fisher information matrix for multidimensional data sets, IEEE Trans. on Sig. Proc., 51(10), 2679—2691.
  • Ober, R. J., Lin, Z., Ye, H., and Ward, E. S. (2002) Achievable accuracy of parameter estimation for multidimensional NMR experiments, J. Mag. Reson., 157, 1—16.
  • Ober, R. J. (2002) The Fisher information matrix for linear systems, Sys. Contr. Letts., 47, 221—226.

Surface Plasmon Resonance (SPR)

  • Ober, R. J., Caves, J., and Ward, E. S. (2003) Analysis of exponential data using a non-iterative technique: application to surface plasmon experiments, Anal. Biochem., 312, 57—65.
  • Ober, R. J. and Ward, E. S. (2002) Compensation for loss of ligand activity in surface plasmon resonance experiments, Anal. Biochem., 306, 228—236.
  • Ober, R. J. and Ward, E. S. (1999) The influence of signal noise on the accuracy of kinetic constants measured by surface plasmon resonance experiments., Anal. Biochem., 271, 70—80. Please see SPRTool for more information.